Use of Bacterial Bioluminescence for Monitoring the Behavior of Rhizobacteria Introduced to Plant Rhizosphere
スポンサーリンク
概要
- 論文の詳細を見る
Bioluminescence (lux) genes from Vibrio harveyi were introduced into an artificial transposon vector pJFF350 as a constitutively expressing lux-genes set. The resultant recombinant plasmid pSOL2 was then transposed into the chromosome of fluorescent pseudomonad strains, in which the lux genes were stably integrated. Root colonization by the lux-marked strains was visually monitored by autophotography, and it was found that the bioluminescence intensities were highly correlated with the populations of the strains colonizing roots. A strain of Pseudomonas putida thus lux-marked was inoculated to spinach seedlings in nursery pots, and the root colonization was assayed. In sterile soil, the strain extensively colonized the entire part of the developing roots and the population densities at any sites were high. In non-sterile soil, only the inoculated site of the roots was weakly colonized. These results suggest that the presence of the indigenous microflora is an environmental constraint on root colonization of the introduced bacteria.
- 社団法人日本土壌肥料学会の論文
著者
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Sakai Masao
Laboratory Of Soil Microbiology Department Of Plant Resources Faculty Of Agriculture Kyushu Universi
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Sakai M
Kyushu Univ. Fukuoka Jpn
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Sakai M
Laboratory Of Soil Microbiology Department Of Plant Resources Faculty Of Agriculture Kyushu Universi
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Matsuguchi T
Laboratory Of Soil Microbiology And Biochemistry Department Of Agricultural Chemistry Faculty Of Agr
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Kim Jong-Shik
Laboratory of Soil Microbiology and Biochemistry, Department of Agricultural Chemistry, Faculty of A
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Matsuguchi Tatsuhiko
Laboratory of Soil Microbiology and Biochemistry, Department of Agricultural Chemistry, Faculty of A
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Futamata Hiroyuki
Laboratory of Soil Microbiology and Biochemistry, Department of Agricultural Chemistry, Faculty of A
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Ozawa Hidenori
Laboratory of Soil Microbiology and Biochemistry, Department of Agricultural Chemistry, Faculty of A
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Sueguchi Tadayoshi
Laboratory of Soil Microbiology and Biochemistry, Department of Agricultural Chemistry, Faculty of A
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Sakai M
Laboratory Of Soil Microbiology Division Of Soil Science And Plant Production Department Of Plant Re
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Sasaki M
Laboratory Of Soil Microbiology And Biochemistry Department Of Agricultural Chemistry Faculty Of Agr
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Sakai Masao
Laboratory Of Soil Microbiology And Biochemistry Department Of Agricultural Chemistry Faculty Of Agr
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Futamata Hiroyuki
Department Of Ecological Engineering Toyohashi University Of Technology:(present Office)department O
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Futamata Hiroyuki
Laboratory Of Soil Microbiology And Biochemistry Department Of Agricultural Chemistry Faculty Of Agr
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Kim Jong-shik
Laboratory Of Soil Microbiology And Biochemistry Department Of Agricultural Chemistry Faculty Of Agr
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Ozawa Hidenori
Laboratory Of Soil Microbiology And Biochemistry Department Of Agricultural Chemistry Faculty Of Agr
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Ozawa Hidenori
Leaf Tobacco Research Laboratory Japan Tobacco Inc.
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Futamata H
Department Of Ecological Engineering Toyohashi University Of Technology
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Sueguchi Tadayoshi
Laboratory Of Soil Microbiology And Biochemistry Department Of Agricultural Chemistry Faculty Of Agr
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Matsuguchi Tatsuhiko
Laboratory Of Soil Macrobiology And Biochemistry Department Of Agricultural Chemistry Faculty Of Agr
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Sakai Masao
Laboratory Of Soil Macrobiology And Biochemistry Department Of Agricultural Chemistry Faculty Of Agr
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