グルコアミラーゼ活性の新規測定法

概要

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A new method for measuring glucoamylase activity is proposed. In this method, G2-CNP (2-chloro-4-nitrophenyl O-α-D-glucopyranosyl-(1→4)-β-D-glucopyranoside) is used as the substrate and the resulting product is measured, from which the amount of enzymatic activity is then quantitatively determined. This method has the following advantages : (i) The presence of glucose in the sample does not influence the results of the measurement, and therefore there is no need to dialyze the sample prior to analysis. (ii) The presence of α-amylase of Aspergillus oryzae in the sample does not affect the results of measurement because it cannot hydrolyze the substrate. (iii) The assay procedure is simple, and can be easily accomplished in a short time. The proposed method showed good correlation with the method generally used for glucoamylase assay. Coefficients of variation were within 1% in both within-day and day-to-day experiments. Since α-glucosidase can also hydrolyze the substrate, the measurement results showed the sum total of glucoamylase and α-glucosidase activities (that is, glucose forming activity) when α-glucosidase was present in the sample. A method for the differential assay of glucoamylase and α-glucosidase activities in the same sample was also developed.
社団法人日本生物工学会の論文
1995-01-25