カルバゾールのクロマトグラフ法による分離と3-メチル-2-ベンゾチアゾリノンヒドラゾンによる吸光光度定量法

概要

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Carbazole can be determined spectrophotometrically by the color development with 3-methyl-2-benzothia-zolinone hydrazone (MBTH). However, this method is difficult to be applied to the determination of small amounts of carbazole in organic substances because the color development is carried out in methanol-water medium. Therefore, chromatographic separation of carbazole from the organic matrix was studied for the application of the MBTH method. Ten milliliters of o-dichlorobenzene solution of the sample was passed through an alumina column (6φ×50 mm), followed by 10 ml of o-dichlorobenzene. The column was washed with 5 ml of pentane. Then, carbazole was eluted with 6 ml of methanol. Five milliliters of 2% solution of ferric chloride in 0.8 M hydrochloric acid was added to the effluent. After the pentane was removed from the solution by evaporation, 5 ml of aqueous solution of MBTH (0.4%) was added. The solution was allowed to stand for 30 min at 25℃, and diluted to 25 ml with the mixture of concentrated hydrochloric acid and methanol (1:1). After standing the solution for 10 minutes, the absorbance was measured at 580 nm against the blank obtained in the same conditions as the sample using 10 ml of o-dichlorobenzene. By this method, 0.004% of carbazole in anthracene, phenanthrene, dibenzofuran and fluorene were determined.
社団法人日本分析化学会の論文
1979-02-05