3-メチル-2-ベンゾチアゾリノンヒドラゾンによるカルバゾールの抽出-吸光光度定量法

概要

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In the presence of ferric chloride, carbazole reacts with 3-methyl-2-benzothiazolinone hydrazone (MBTH) to form a colored product in methanolwater medium, and the product can be effectively extracted with chloroform. The reagent blank was influenced by the concentration of ferric chloride. In order to stabilize the reagent blank, it was necessary to stand after addition of HCl-CH_3OH mixture, and also after extraction. To 5 ml of methanol solution of the sample, 5 ml of 1 % solution of ferric chloride in 0.8 M hydrochloric acid and 5 ml of 0.4 % aqueous solution of MBTH were added. The solution was allowed to stand for 45 min at room temperature and diluted to 25 ml with a mixture of concentrated hydrochloric acid and methanol (1:1). After standing for 20 min, the colored product was extracted with 5 ml of chloroform. After complete separation of the two phases, the chloroform phase was stood for 20 min. Then, the absorbance of the chloroform extract was measured at 625 nm against the reagent blank. The calibration curve was linear in the range of 0.5 μg to 10 μg of carbazole.
社団法人日本分析化学会の論文
1980-12-05