モノグアニルメラミンの陽イオン交換樹脂による分離と紫外吸收による定量

概要

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Monoguanylmelamine was separated from its related compounds on a cation exchange column (AG-50WX4 in Na-form, 8φ×300 mm) by stepwise elution with Sorensen's buffer solution (pH 10.2〜10.4 and pH 11.6〜11.8) at the flow rate of 1.5 ml/min and the column temperature 30℃. The interfering compounds such as melamine, acetoguanamine, acetoguanide, ammeline, acetoguanamide and cyanomelamine were completely removed from the column by (100〜150) ml of the first buffer solution (pH 10.2〜10.4) and then, monoguanylmelamine was eluted by (50〜70) ml of the second buffer solution (pH 11.6〜11.8) without interference of diguanyimelamine. Quantitative determination of monoguanylmelamine was performed by calibrating the peak area of chromatogram monitored by the absorbance at 255 nm. Monoguanylmelamine could be determined down to 5 μg by the proposed method.
社団法人日本分析化学会の論文
1976-02-10