ブロモクロロフェノールブルーを用いる微量界面活性剤のセルロース粉末予備濃縮/吸光光度定量

概要

論文の詳細を見る
A simple method for the spectrophotometric determination of trace surfactants based on a preconcentration technique involving collection on a cellulose powder was developed. A cationic surfactant (CS^+), such as quaternary ammonium salts containing a long-chain alkyl group, was collected on a cellulose powder as a 1 : 1 ion associate with a monovalent Bromochlorophenol Blue anion (HBCPB^-). For the anionic surfactant (AS^-), CS^+ was added to AS^- and similarly the ion associate of excess CS^+ with HBCPB^- was collected on cellulose powder. After filtration, the cellulose powder with an ion associate, CS^+・HBCPB^-, was stirred with a small volume of an alkaline solution. The absorbance of divalent BCPB^2- in the solution after centrifuging was measured at 590 nm. The absorbance at 590 nm increased and decreased with increasing the amount of CS^+ and AS^-, respectively. A calibration graph based on this principle shows linearity up to 5×10^-7 M of CS^+ and AS^-. The proposed method is simple and rapid, and there is no use of toxic organic solvents.
2002-03-05