Streptomyces olivaceusによるビタミンB_<12>の生産 : (VII) ビタミンB_<12>の精製に関する研究

概要

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The purification of vitamin B_<12> was undertaken in the culture broth of Streptomyces olivaceus and the vitamin was obtained in crystalline forms. Activated charcoal was found to be the most potent adsorbing agent, and conditions of adsorption and elution were investigated. Aqueous solutions of propanol, butanol, benzyl alcohol, acetone and pyridine were found to be exceeding eluting agents. As the intermediary purification process, solvent extraction method with phenol-butanol gave satisfactory results. Resin chromatography with Amberlite IRC-50 was applied as the final purification process wherein a washing with neutral aqueous acetone was found to improve the recovery of pure chromatographic fractions. Purity improvements in purification processes were traced by the changes of absorption curves.
日本ビタミン学会の論文