Propionibacterium shermaniiによるコリノイドおよびその補酵素型の生合成経路, とくに補酵素化の時期

概要

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The biosynthetic pathway of corrinoids and their coenzyme-forms in P. shermanii was studied. Resting cells of P. shermanii of 4 day culture were incubated under anaerobic condition for 24 hours with δ-aminolevulinic acid in the presence of cobalt sulfate. Incomplete corrinoids obtained were about 65% of the total corrinoids formed. Cobinamide, mono-to penta-carboxylic acids of incomplete corrinoids, and their coenzyme-forms were separated by paper ionophoresis. Examination after chemical amidation of the carboxylic acids obtained showed that di-to tri-amides of cobyrinic acids were predominant. The result sobtained suggest that the biosynthesis of corrinoids seems to proceed from cobyrinic acid via cobyric acid to cobinamide, and 5'-deoxyadenosyl group might be incorporated to cobalt atom in the corrin ring, at latest stage of cobyrinic acid diamide formation.
日本ビタミン学会の論文
1970-07-25