微生物による炭化水素の利用 : (I) 数種細菌によるn-パラフィンの資化とビタミンB_<12>生産性

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Pseudomonas (Ps.) aureofaciens, Ps. ovalis and Ps. aeruginosa were studied on their utilization of n-paraffins as well as vitamin B_<12> Production in comparison with Nocardia gardneri and Mycobacterium smegmatis already reported. It seems difficult to correlate the types of microorganisms and those of n-paraffins tasted (in regard to the hydrocarbon utilization and vitamin B_<12> production). It was found that the majority of vitamin B_<12> existed in the culture fluid when cultured on a simple n-paraffin medium. More detailed investigations were made on the effects of culture conditions using Ps. aeruginosa. The addition of an appropriate nitrogen source, especially a natural one such as cassamino acid, polypeptone, or yeast-extract, or ammonium acetate promoted the utilization of n-parafiins and vitamin B_<12>. Substitution of n-paraffin as a single carbon source in the medium for a n-paraffin mixture resulted in higher hydrocarbon utilization and vitamin B_<12> production. The addition of one of certain non-ionic detergents promoted the vitamin production, but it had no effect on the retention of the vitamin in the cells. Cobalt sulfate and 5,6-dimethylbenzimidazole were effective as precursors of the vitamin.
社団法人日本生物工学会の論文
1969-08-25