炭化水素醗酵の生物化学工学的研究 : (第5報)炭化水素資化性酵母の対酸素挙動の解析
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Some aspects of the behavior of Candida petrophilum, a hydrocarbon assimilating yeast, during respiration and oxygenation of n-paraffins, toward oxygen in a dissolved state, have been investigated employing a method for analyzing the attenuation curves of dissolved oxygen as proposed by Terui and Konno.The yeast was cultivated in a jar fermenter on n-hexadecane with a dissoleved oxygen concentration of 5 to 6 mg/l. The fits of the relationship between the respiration rate and dissolved oxygen for the Michaelis-Menten function has been found to be fairly good with aerobically grown cells of the yeast.Two kinds of K_m values for the dissolved state of oxygen were observed. The oxygenterminal entity with a higher affinity for dissolved oxygen has a K_m value of 1 to 2×10^<-5> M/l, while that with a lower affinity has a K_m value of 2 to 3×10^<-4> M/l.Some aspects of the behavior of the enzyme systems concerned with the oxygen consumption of cells growing on n-hexadecane were analyzed by experiments using a respiratory inhibitor, NaN_3.Three kinds of enzyme systems for oxygen were found to be operating. The first was the cytochrome system which is sensitive to NaN_3 and has an apparent K_m value of 1 to 2×10^<-5> M/l. The second system was the oxygenase system which catalyzes the oxygenation of n-paraffins. This is insensitive to NaN_3 and has a K_m value of 5.1 to 7.4×10^<-5> M/l. The third enzyme system is insensitive to NaN_3. The K_m value is 4.0 to 5.2×10^<-4> M/l, and it may be composed flavin proteins.Affinity of these enzyme systems for oxygen was much affected by the dissolved oxygen concentration around the cells. Cells grown with 0 mg/l of dissolved oxygen had no clear cytochrome system, but they could adapt toward oxygen within one or two hours when the yeast was cultivated with more than 1 mg/l of dissolced oxygen. With 0.2 to 0.1 mg/l of dissolved oxygen, it took more than four hours for the respiratory enzyme system to appear completely. Higher dissolved oxygen concentration (10 mg/l) depressed the growth of the yeast, and eliminated totally the K_<m2> component of the respiratory systems.
- 社団法人日本生物工学会の論文
- 1972-05-25
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