醤油醸造に関与する耐塩性乳酸菌に関する研究 : (第3報) Tetracoccus No.100の耐塩性に対するL-Proline効果の酵素レベルにおける解析

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The characteristic effect of L-proline on the halotolerance of Tetracoccus No. 100,reported in a previous paper, was further studied with several enzymes. The results presented in this paper are as follows : 1. Among the stereoisomers and analogs of proline, such as L-proline, D-proline, hydroxy-L-proline, pyrrole, pyrrolidine, and L-pyroglutamic acid, L-proline was the sole compound facilitating the halotolerance of Tetracoccus No. 100.2. The activity of FDP-aldolase (fructose-1,6-diphosphate-aldolase), depressed by a high concentration of NaCl, was partly recovered by the addition of L-proline.3. Regardless of the salt concentration, L-proline showed no effect on the action of NAD-linked lactic dehydrogenase and flavin-linked lactic dehydrogenase, both enzymes being salt sensitive.4. The specific activity of NAD-linked lactic dehydrogenase from cells grown on L-proline-enriched hypertonic saline medium was several times higher than that of normally grown cells. This fact strongly suggests that L-proline is concerned in the formation of this enzyme.5. The catalase activity of Tetracoccus No. 100 was partly inhibited by salt concentrations above 10 per cent, and was completely recovered by the addition of 10 mM L-proline.
社団法人日本生物工学会の論文
1971-10-25