山廃酒母の亜硝酸生成菌に関する研究 : (第1報)特異な有用細菌群の分離

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The microflora in yamahaimoto or sake making starter mash was investigated.It was found that there was a difference between the microflora in nitrite rich yamahaimoto and that in nitrite poor. In nitrite rich yamahaimoto a specific bacteria was observed to dominate during the nitrite forming period.For isolation and enumerating of the specific bacteria, K' medium was prepared by extracting rice-koji with water at low temperature (5℃) for one day and sterilized with Seitz-filter.The distinguishing character of the specific bacteria was that they could grow at 0℃ for one week and maximally at 22℃ under anaerobic conditions with NO^-_3. It was therefore assumed to be an obligate psychrophilic nitrate respirator.The specific bacteria were polar flagellated, gram staining negative, rod shaped and lactose fermentation negative. They produced soluble pigment of green fluorescence, and attacked p-hydroxybenzoate and other benzoic acid derivatives. They were classified as belonging to the genus Psudomonas.The authors were able to successffuly stabilize and enlarge the yamahaimoto by adding this bacteria.
社団法人日本生物工学会の論文
1967-09-25