山廃酒母の亜硝酸生成菌に関する研究 : (第5報) Pseudomonas sp. 37330-0株の硝酸還元酵素系への米麹成分の働らき

概要

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Nitrate reducing acitivity of diluted cell suspension or cell-free extract of Pseudomonas sp.(strain 37330-0) isolated from "Yamahaimoto" was increased in the presence of rice-koji or koji-extract. It was found that the nitrate seducing activity s-as specifically increased in the presence of L-malic acid or fumaric acid in koji-extract and that L-malic acid was the most effective substance so far tested. It was also found that koji-extract served as an electron donor in the reduction of NADP but not in the reduction of NAD by cell suspension or sonicate.Only L-malic acid and fumaric acid in koji-extract were found to be effective in the reduciton of NADP and it has been observed that no other compounds so far studied could serve as electron in the reduction of nitrate so effectively as L-malic acid. It was concluded that the specific activation of nitrate reducing system of this strain by rice-koji or koji-extract was mainly due to the presence of L-malic acid in koji-acting as an electron donor to NADP.A possible model for the nitrate reducing system of this was presented, in which electron was transferred as the following sequence : L-malic acid → NADP → menadione → cyt. c → nitrate reductase.
社団法人日本生物工学会の論文
1971-05-25