放線状菌の分類に關する研究(第1報) : 培養及び鏡檢方法

概要

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We devised a new method for culture and microscopic observation of Streptomycetaceae and Actinomycetaceae. This method can be applied to other filamentous fungi. The outline of this method is as follows.Two cover rlasses are affixed, putting small this papers between the glasses at the four corners. One edge of this cover glass set is soaked in a melted agar medium which will be absorbed into the space between glasses. When absorbed till about half altitude, the set is removed and cooled . After the agar medium coagulated, the cover glass set is stuck into beforehand coagulated agar in a vessel. And then, with a platinum loop, spore suspension is inoculated on the outside of te upper side of agar medium inside the cover glasses. The spore suspension will be absorbed and spread along the upper edge of coagulated agar medium. Putting this inoculated set in a suitable coverd cup, incubate at a given temperature, so the inoculated microorganism will grow on the upper side of coagulated agar medium in the cover glasses.After the growth of microbe the cover glass set is removd from stuck agar holder and is put on the slide glass under the object glass of microscope.
社団法人日本生物工学会の論文
1953-12-15