放線状菌の分類に關する研究 (第2報) : 氣菌糸形成用培地の選定

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We researched a proper medium for microscopic observation of Streptomycetaceae. After various experiments, we decided the most suitable series of media consisting of media which we call C.B. series media. By using these series media and cover culture method we can conquer some difficulties that occur on microscopic observation of Streptomycetaceae.These series media consist of glucose, beef extract, peptone, potassium phosphate (dibasic), magnesium sulphate and potassium chloride. The four media of this series are the same in the components as above, but different in the concentration of glucose, beef extract and peptone, as follows ; 1) glucose 1%, beef extract 0.5%, peptone 0.5%, 2) glucose 0.5%, beef extract 0.2%, peptone 0.2% 3) glucose 0.2% beef extract 0.1%, peptone 0.1%, 4) glucose 0.1%, beef extract 0.05%, peptone 0.05%, and the mineral salts of each medium are the same as follows, K_2HPO_4 0.1%, MgSO_4・7aq. 0.05% and KCl 0.05%.When these four media are used for the culture of Streptomycetaceae, many strains form on some one or more of them aerial mycelia ehich show the complete morphological characteristics of the strains.
社団法人日本生物工学会の論文
1954-06-15