Non Boil Radiodilution Assayによる血清ビタミンB_<12>・葉酸同時測定法の基礎的並びに臨床的検討

概要

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The simultaneous radiodilution assay of serum vitamin B_<12> and folate was studied on the so-called "non boil methods", in which these two vitamins were released from their endogenous binding proteins with alkaline denaturation and separated the bound vitamins from the free ones with the magnetic iron particles coated these vitamin binders (purified hog intrinsic factor and β-lactoglobulin from cow milk), compared with other radiodilution assays. 1. The reproducibilities of this method were found 3.42〜5.25% of CV on B_<12> and 3.68〜7.89% on folate, with satisfaction. 2. The recovery tests of the two serum vitamins with the use of CN-cobalamin and folic acid (J.P.) were finely showed the ratios of 99.4〜100.4% on B_<12> and 99.3〜101.8% on folate, respectively. The values of both vitamins measured by this method were found slightly lower than those by boil method, and higher than those by non boil charcoal absorption method. 3. Normal range of serum total B_<12> concentration was 229〜847 (465±155, n=325) pg/ml and that of folic acid was 2.34〜7.08 (4.23±1.19, n=310) ng/ml. 4. The values of serum B_<12> measured by this method were found remarkably low in malignant lymphoma cases with gastorectomy during antitumor drug therapy, compared with its hematological findings of bone marrow.
日本ビタミン学会の論文
1990-10-25