けい光分析法による脂溶性ビタミン類の定量 : (I)α-トコフェロール酸化生成物のけい光スペクトル

概要

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In order to establish the fluorometry of tocopherols, dl-a-tocopherol acetate was saponified by potassium hydroxide with manganese dioxide. Strong fluorescence was observed on the unsaponifiable matter which showed a maximum at 385 mμ. Fractionating the fluorescent substances by thin layer chromatography, and the most conspicuous fluorescent spot was extracted with benzene from the chromatogram. The fluorescent spectra was identical with that of the original unsaponifiable matter. Linear relationship was noted between the amount of the unsaponifiable matter in benzene and the intensity of fluorescence at 385 mμ if the original dl-a-tocopherol acetate was limited under 0.3 mg per ml of the benzene. These facts suggest a possibility to determine the tocopherols fluorometrically.
日本ビタミン学会の論文
1969-01-25