共晶中での酵母の保存と凍結保護物質の効果 : 酵母の凍結障害に関する研究(第6報)

概要

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Two types of freeze-thaw damage caused by eutectic freezing and long-period storage were studied. In long storage, more cells survived rate in the eutectic mixture of electrolyte than in the solution. The survival of cells was low when the cell suspension was stored slightly below the eutectic temperature in the eutectic mixture of KCl, Na_2SO_4,or KNO_3 which have high eutectic temperatures. The FID curve of the eutectic solution showed a little response of water slightly below te eutectic temperature, indicating incomplete eutectic crystallization. The addition of a cryoprotective compound such as glucose and DMSO interfered with complete crystallization of the eutectic solution and decreased the freeze-thaw damage caused by eutectic freezing, In long-period storage, glucose was the most effective cryoprotective additive among the compounds tested.
社団法人日本生物工学会の論文
1985-01-25