グルコシル受容体としての水とエタノールの選択性 : β-グルコシダーゼによるセロビオースからのエチルβ-グルコシドの生成(第1報)

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The β-glucosidase from Trichoderma viride (Meicelase P-1) has glucosyltransferase activity. In this study, the effects of the alcohol concentration on the ethyl-β-glucoside formation by the β-glucosidase were investigated to study the selectivity between water and ethanol as the glucosyl-accerator. Cellobiose was used as the blucosyl-donor. There were two different main reactions. One is reaction 1 in which ethyl-β-glucoside (1 mole) and glucose (1 mole) are formed from cellobiose (1 mole) and ethanol (1 mole), the other is reaction 2 in which 2 moles of glucose are formed from cellobiose (1 mole). The results obtained were as follows. With the increase of alcohol concentration, ethyl-β-glucoside was increased. When the substrate existed, the ratio of progression of reaction 1 to reaction 2 was constant at alcohol concentrations from 10 to 60%. The selective ratio of reaction 1 was about 50 times that of reaction 2. The activity of the β-glucosidase is lower at higher alcohol concentratioins (50 or 60%). After the substrate was consumed, the hydrolysis of ethyl-β-glucoside became predominant. At lower alcohol concentrations, oligosaccharides such as cellotriose were temporarily formed but disappeared as with the reaction proceeded.From the above facts, we concluded that in the glucosyltransfer reacton by β-glucosidase, the preference for ethanol over water as the glucosyl-accepter was about 50 times higher and independent of the ethanol concentration.
1990-01-25

グルコシル受容体としての水とエタノールの選択性 : β-グルコシダーゼによるセロビオースからのエチルβ-グルコシドの生成(第1報)

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