植物ウイルスの虫媒伝染機作に関する研究 (第8報) : 放射性同位元素P^<32>による萎縮病温州柑およびアオバハゴロモの燐酸代謝の観察

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In the previous reports, abnormal phosphorous metabolisms were observed in both the dwarf virus-infected mandarin plant and the virus transmitting-green broad winged planthopper. This paper deals with the experiments to elucidate the rate of incorporation of P^<32> into the phosphorous fractions in these abnormal metabolisms. (1) The radioactivity of P^<32> in various phosphorous fractions of mandarin plant or planthopper shows relatively higher level, as affected with the virus (Tables 1〜2). (2) The acid soluble phosphorous compounds of the examined material were fractionated by the method of LePage and Umbreit. In both the plant and the insect, the amounts of phosphorous contents in the various fractions show no consistent difference in trend between healthy and diseased individuals. The radioactivity in each fraction of the viruliferous insect is, however, found on considerably higher level than that of the nonviruliferous one (Tables 3-4 and Figs. 1-2). (3) The nucleic acids examined were extracted b the method of Ogur-Rosen and Schneider from orange plant, and by the Schneider's method from planthopper. The incorporation of P^<32> into the nucleic acids is considerably lower rate, and the autoradiograms present no difference between healthy and diseased plant or between nonviruliferous and viruliferous planthopper, respectively (Figs. 3〜4). It is suggested that the nucleic acid is to be prepared a more suitable technique under milder conditions.
日本植物病理学会の論文
1959-09-30

植物ウイルスの虫媒伝染機作に関する研究 (第8報) : 放射性同位元素P^<32>による萎縮病温州柑およびアオバハゴロモの燐酸代謝の観察

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