タバコ・モザイク・ウイルスに感染したNicotiana glutinosa葉の局部病斑形成時期における蛋白質および核酸代謝

概要

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Protein and nucleic acid metabolisms in tobacco mosaic virus-infected Nicotiana glutinosa leaves were investigated. The plants were incubated at 32 C for 2 or 3days after inoculation with tobacco mosaic virus to establish a systemic infection and then some of these infected plants were transferred to 25 C to induce local lesions and the others were kept 32 C as controls. The leaves of each plant were detached and labele by ^<14>CO_2. The supernatant fraction(S), mainly soluble proteins, obtained from the sap of the leaves by ultracentrifugation (105,000 × g) and the SDS-solubilized fraction (PS), mainly particulate-bound proteins, of precipitates at 10,000 × g, were electrophoresed on polyacrylamide gel columns. No difference in the proportion of protein was detected in both S and PS fractions between control and shifted plants. The incorporation of ^<14>C from ^<14>CO_2 into the pellet fraction (P) at 105,000 x g, virus fraction, was reduced by the temperature shift. Methylated albumin kieselgur column chromatography of nucleic acid fractions obtained by phenol-SDS method showed that no significant change occurred in the proportion of molecular species of nucleic acids after temperature shift.
日本植物病理学会の論文
1978-04-25