サトウキビプロトプラストの分離, 細胞融合及び培養に関する研究

概要

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Protoplasts were isolated from callus and plantlets regenerated from the same callus of sugarcane by treatment with 0.1% Pectolyase Y-23, 3% Cellulare YC under aseptic conditions. The protoplasts from each source were fused and cultured in liquid medium. Fluorescence microscopic studies showed that more than 85% of the freshly isolated protoplasts were viable. Calcofluor White was used to demonstrate the regeneration of cell walls in these protoplasts. Non-fused protoplasts and fused protoplasts isolated from callus regenerated cell walls by 2 h and their cell divisions were observed in 4 days after culture. Non-fused protoplasts and fused protoplasts from plantlets regenerated cell wall by 1 day and cell divisions were observed in 5 days after culture. The fused protoplasts between callus protoplasts and leaf protoplasts regenerated cell walls by 1 day and cell divisions were observed in 5 days after culture.
1993-06-01