Transformation of the White-rot Basidiomycete Coriolus hirsutus Using the Ornithine Carbamoyltransferase Gene(Biochemistry & Molecular Biology)
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概要
- 論文の詳細を見る
An efficient transformation system for the basidiomycete Coriolus hirsutus was developed. A double-auxotrophic mutant of C. hirsutus, deficient both in ornithine carbamoyltransferase (OCTase) and 3-isopropylmalate dehydrogenase (3-IPM dehydrogenase), was transformed to Arg^+ with each allelic type of the C. hirsutus genomic OCTase gene (arg1) newly cloned. The transformation frequency of 10^3-10^4 transformants per μg DNA per 10^6-10^7 oidial protoplasts was reached. Southern blots showed that the transforming DNA was integrated into chromosomal DNA with multi-copies. The Arg^+ phenotype of the transformants was stably inherited through mitosis.
- 社団法人日本農芸化学会の論文
- 2003-10-23
著者
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TSUKAMOTO Akira
Advanced Research Laboratory, Hitachi, Ltd.
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Tsukamoto Akira
Advanced Research Laboratory Hitachi Ltd.
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Shouguchi Junko
Department Of Life Science Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technology
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Tsukamoto Akira
Advanced Technology Research Laboratory Oji Paper Co. Ltd.
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Sugiura J
Advanced Technology Research Laboratory Oji Paper Co. Ltd.
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Sugiura Jun
Advanced Technology Research Laboratory Oji Paper Co. Ltd.
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KOJIMA Yasushi
Advanced Technology Research Laboratory, Oji Paper Co., Ltd.
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KITA Yukio
Advanced Technology Research Laboratory, Oji Paper Co., Ltd.
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Kita Yukio
Advanced Technology Research Laboratory Oji Paper Co. Ltd.
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Kojima Yasushi
Jaxa
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