Lignin-Degrading Enzyme Production by Bjerkandera adusta Immobilized on Polyurethane Foam

概要

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Production of the lignin-degrading enzymes lignin peroxidase(Lip), manganese peroxidase(MnP), and laccase(Lac)by the white-rot fungus Bjerkandera adusta was investigated experimentally using polyurethane foam(PUF)as a carrier of immobilized fungal mycelia. An immobilized cell culture with a low-nitrogen medium yielded significantly greater LiP, Mnp, and Lac activities in comparison with those obtained in a liquid culture. The maximum activities of the three enzymes were 450, 370, and 100 U/ml, respectively, under the following incubation condition : glucose concentration, 20 g/l ; temperature, 30℃ ; pH 4.5. The activities of MnP and Lac were significantly higher than those reported using other incubation methods. Lignin was degraded to the extent of 40% and its decolorization ratio was about 70% at an incubation time of 40h using lignin-degrading enzymes from B.adusta. Six different isozymes of MnP were synthesized by B.adusta, two of which exhibited high MnP activity. Our preliminary finding that extracellular enzymes from B.adusta are capable of degrading and decoloring lignin makes these enzymes attractive for further research aimed at their large-scale application in lignin depolymerization, pulp biobleaching, and the degradation of toxic pollutants.
社団法人日本生物工学会の論文
1999-07-25