Lignin Peroxidase Production by Phanerochaete Chrysosporium Immobilized on Polyurethane Foam.
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概要
- 論文の詳細を見る
Production of lignin peroxidase by a white-rot basidiomycete, <I>Phanerochaete chrysosporium</I>, was investigated experimentally using polyurethane foam as a carrier of immobilized fungal mycelia. The immobilized cell culture using polyurethane foam as a carrier of mycelia yielded significantly greater lignin peroxidase activity than the conventional stationary liquid culture. The effects of operational conditions, such as the kind and number of polyurethane foam cubes, glucose concentration and temperature, on the lignin peroxidase production were examined. Addition of 0.05% Tween 80, 1 mM veratryl alcohol and 1 mM FeSO<SUB>4-</SUB>·7H<SUB>2</SUB>O greatly improved the production of lignin peroxidase up to 2,700 units/ml culture medium. The lignin peroxidase activity in this culture was about three times larger than that obtained from the culture cultivated in the absence of these additives. Step change incubation lowering the temperature from 37°C to 30°C over an incubation time of three days was carried out for the large scale production of lignin peroxidase, and this incubation gave the highest lignin peroxidase activity 3,800 units/ml culture medium.
- The Society of Chemical Engineers, Japanの論文
著者
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Kobayashi Fumihisa
Department Of Chemistry And Chemical Engineering Faculty Of Engineering Kanazawa University
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SUNGUSIA Mutui
Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University
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SAWADA Tatsuro
Department of Chemistry and Chemical Engineering, Faculty of Engineering, Kanazawa University
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Nakamura Yoshitoshi
Department Of Chemistry And Chemical Engineering Faculty Of Engineering Kanazawa University
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Sawada Tatsuro
Department Of Applied Mathematics Faculty Of Engineering Science Osaka University
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Kuwahara Masaaki
Wood Research Institute Kyoto University
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Ito Hiromichi
Wood Research Institute of Kyoto University
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