Expression of Cytochrome c-553(CO) Gene That Complements the Second Subunit Deficitncy of Membrane-Bound Alcohol Dehydrogenase in Gluconobacter suboxydans subsp. α

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概要

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• Cytochrome c-553 (CO) gene was subcloned onto a shuttle vector pGEA1 able to replicate in Gluconobacter suboxydans and Escherichia coli, forming pGEAC1.pGEAC1 transformed G. suboxydans subsp. a which has low ethanol oxidation activity because of a second subunit deficiency of membrane-bound alcohol dehydrogenase (ADH). The transformants harboring pGEAC1 expressed theanol oxidation activity and produced matirial cross-reactive with anti-cytochrome c-553 (CO) antibody in the membrane. Furthermore, expression of cytochrome c-553 (CO) gene resulted in elevated levels of heme c and CO-reactive cytochrome c, complemented the deficiency of the second ADH subunit, and restored ethanol oxidase activity. We previoulsy reported that cytochrome c-553 (CO) has significant amino acid sequence homology (60%) to the cytochrome subunit of ADH from Acetobacter aceti (Takada, Y. et al., J. Ferment. Bioeng., 72,1,1991). Hence, cytochrome c-553 (CO) is the second subunit of ADH.

• 社団法人日本生物工学会の論文
• 1992-02-25

著者

• Shimizu Toshio

  Foods Research Group, Foods Division, Asahi Chemical Industry Co., Ltd.

• Takeda Yasuhiko

  Foods Research Group, Foods Division, Asahi Chemical Industry Co., Ltd.

• Takeda Yasuhiko

  Foods Research Group Asahi Chemical Industry Co. Ltd.

• Shimizu Toshio

  Foods Research Group Asahi Chemical Industry Co. Ltd.

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• Expression of Cytochrome c-553(CO) Gene That Complements the Second Subunit Deficitncy of Membrane-Bound Alcohol Dehydrogenase in Gluconobacter suboxydans subsp. α
• Comparison of the Second Alcohol Dehydrogenase Subunit Gene in Acetic Acid Bacteria
• Cloning and Sequencing of the Gene Encoding Cytochrome c-553 (CO) from Gluconobacter suboxydans
• Role of Cytochrome c-553(CO), the Second Subunit of Alcohol Dehydrogenase, in the Azide-Insensitive Respiratory Chain and in Oxidative Fermentation of Gluconobacter Species

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