Cloning and Sequencing of the Gene Encoding Cytochrome c-553 (CO) from Gluconobacter suboxydans
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概要
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The gene encoding cytochrome c-553 (CO) from Gluconobacter suboxydans was cloned by using a synthetic oligonucleotide probe. The complete nucleotide sequence of the cytochrome c-553 (CO) gene including 5' and 3' flanking regions has been determined. The sequence predicts a 478 amino acid residue protein product which contains three Cys-X-Y-Cys-His heme c binding sites. The predicted amino acid sequence of the cytochrome c-553 (CO) gene was found to have considerable similarity (60%) to that of the alcohol dehydrogenase (ADH) cytochrome subunit from Acetobacter aceti. It is suggested that the cytochrome c-553 (CO) may act as an ADH cytochrome subunit for ethanol oxidation and be related to the KCN-insensitive and non-energy-generating bypath in the sugar-oxidizing respiratory chain in G. suboxydans.
- 社団法人日本生物工学会の論文
- 1991-07-25
著者
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Shimizu Toshio
Foods Research Group, Foods Division, Asahi Chemical Industry Co., Ltd.
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Takeda Yasuhiko
Foods Research Group, Foods Division, Asahi Chemical Industry Co., Ltd.
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Takeda Yasuhiko
Foods Research Group Asahi Chemical Industry Co. Ltd.
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Shimizu Toshio
Foods Research Group Asahi Chemical Industry Co. Ltd.
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