Extracellular Lipopolysaccharide Production by Erwinia carotovora
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概要
- 論文の詳細を見る
The plant-pathogenic becterium Erwinia carotovora FERM P-7576 produced lipopolysaccharide (LPS) in culture broth. A combination of two carbon sources, such as pectin and other carbohydrates, promoted LPS production. The organism produced the LPS during exponential growth. Production of the LPS was influenced by the cultivation method and temperature. About 920 mg per l of LPS was obtained by two-stage cultivation, in which the bacterium was grown on pectin medium at the end of the exponential growth phase, then glycerol and sodium L-glutamate were added to continue the cultivation. The maximum productivity was obtained at 33℃. The extracellular LPS was essentially identical to the cellular LPS isolated from the cells, on the basis of chemical compositions, as analyzed by sodium dodecylsulfate polyacrylamide gel electrophoresis, and endotoxic activity.
- 社団法人日本生物工学会の論文
- 1989-11-25
著者
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SODE Koji
Department of Biotechnology, Tokyo University of Agriculture and Technology
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KARUBE Isao
Research Center for Advanced Science and Technology, The University of Tokyo
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Sode Koji
Dept.biotechnolgy & Life Science Tokyo University Of Agriculture And Technology.
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Sode Koji
Research Center For Advanced Science And Technology University Of Tokyo
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Karube I
Tokyo Univ. Technol. Hachioji‐shi Jpn
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Karube Isao
Research Center For Advanced Science And Technology The University Of Tokyo
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Karube I
Research Center For Advanced Science And Technology University Of Tokyo:research Laboratory Of Resou
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FUKUOKA SATOSHI
Government Industrial Research Institute
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KAMISHIMA HIROSHI
Government Industrial Research Institute
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Kamishima Hiroshi
Goverment Industrial Research Institute Sikoku
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Sode K
Dept.biotechnolgy & Life Science Tokyo University Of Agriculture And Technology.
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Kamishima H
Government Industrial Research Inst. Shikoku Takamatsu Jpn
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Karube Isao
Research Center For Advanced Bionics National Institute Of Advanced Industrial Science And Technolog
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