Preparation and Properties of Immobilized Mercuric Reductase in Porous Glass Carriers

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概要

• 論文の詳細を見る

• The immobilized mercuric reductase covalently coupled to porous glass was prepared and its properties were investigated. Mercuric reductase immobilized on arylamino and carboxyl derivatives exhibited high relative activities about 20-30% compared with soluble enzymes. The optimum pH of mercuric reductase immobilized on these derivatives was 7.5, slightly higher than that of soluble state (7.0-7.2). The stability of mercuric reductase increased with immobilization onto arylamino derivative and the immobilized enzyme maintained at 30℃ for 29 days showed 80-90% of initial activity. As the application of immobilized enzyme, mercuric ion sensor was prepared and it was found that mercuric ion higher than 0.5μM was detectable with this sensor.

• 社団法人 日本セラミックス協会(The Ceramic Society of Japan)の論文

著者

• Tamiya E

  Graduate School Of Engineering Osaka Univ. 2-1 Yamadaoka Suita Osaka 565-0871 Jpn

• Karube I

  Tokyo Univ. Technol. Hachioji‐shi Jpn

• Karube Isao

  Research Center For Advanced Science And Technology The University Of Tokyo

• Tamiya Eiichi

  Japan Advanced Institute For Science And Technology Hokuriku

• Karube I

  Research Center For Advanced Science And Technology University Of Tokyo:research Laboratory Of Resou

• Suzuki Masayasu

  Faculty Of Computer Science And Systems Engineering Kyushu Institute Of Technology

• Suzuki M

  Kyushu Inst. Technol. Fukuoka Jpn

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