Basic Studies on Packed Bed Reactor Using Aminoacylase-pellets
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概要
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Aminoacylase was immobilized on the mycelium pellet of Aspergillus ochraceus by using albumin and glutaraldehyde. Aminoacylase-pellets (147 units/g wet cells) were applied to a reactor system and continuous hydrolysis of acetyl-L-methionine was carried out. Hydrolysis of acetyl-L-methionine decreased both with increasing space velocity and with increasing substrate concentration. Acetyl-L-methionine was completely hydrolyzed at a concentration of less than 0.08 M by the recycle reactor system. The half-life of aminoacylase-pellets in the reactor was 41 days. Kinetics of the amino-acylase-pellet column were studied. The integrated Michaelis-Menten's equation could be applied to the aminoacylase-pellet column. The apparent Michaelis constant was decreased with increase in space velocity. On the other hand, the rate constant was increased with increasing space velocity.
- 公益社団法人日本生物工学会の論文
- 1977-08-25
著者
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Hirano Ken-ichi
(present Address)amano Pharmaceutical Co. Nishiharumachi Nishikasugaigun Aichi-ken.
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Karube Isao
Research Center For Advanced Bionics National Institute Of Advanced Industrial Science And Technolog
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MATSUNAGA TADASHI
Research Laboratory of Resources Utilization, Tokyo Institute of Technology
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SUZUKI SHUICHI
Research Laboratory of Resources Utilization, Tokyo Institute of Technology
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Suzuki Shuichi
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
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Matsunaga Tadashi
Research Laboratory Of Resources Utilization Tokyo Institute Of Technology
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