Photocontrol of Trypsin-spiropyran Membrane Activity

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概要

• 論文の詳細を見る

• Trypsin (EC 3.4.4.4) was immobilized in collagen membrane modified with β-1-[3,3-dimethyl-6'-nitrospiro(indoline-2,2'-2H-benzopyran)] propionic anhydride. The trypsin-spiropyran (collagen) membrane showed reverse photochromism. The specific activity of trypsin in the spiropyran membrane was 10% of that of native trypsin. The activity of the trypsin-spiropyran membrane under visible light was decreased to 78% of that in the dark. The optimum pH of the trypsin-spiropyran membrane was higher than that of native trypsin. The apparent Michaelis constant (K_m') of the trypsin-spiropyran membrane in the dark ws not changed with visible light irradiation. The apparant diffusion coefficient of the substrate in the dark was larger than that under visible light.

• 公益社団法人日本生物工学会の論文
• 1977-08-25

著者

• Karube Isao

  Research Center For Advanced Bionics National Institute Of Advanced Industrial Science And Technolog

• Nakamoto Yoshiaki

  Faculty Of Engineering Depertment Of Industrial Chemistry University Of Kanazawa

• TERAWAKI SEIKICHI

  Faculty of Engineering, Depertment of Industrial Chemistry, University of Kanazawa

• NISHIDA MORIE

  Faculty of Engineering, Depertment of Industrial Chemistry, University of Kanazawa

• SUZUKI SHUICHI

  Research Laboratory of Resources Utilization, Tokyo Institute of Technology

• Nishida Morie

  Faculty Of Engineering Depertment Of Industrial Chemistry University Of Kanazawa

• Terawaki Seikichi

  Faculty Of Engineering Depertment Of Industrial Chemistry University Of Kanazawa

• Suzuki Shuichi

  Research Laboratory Of Resources Utilization Tokyo Institute Of Technology

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