Amperometric Glucose Sensor Using Thermostable Co-Factor Binding Glucose Dehydrogenase
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概要
- 論文の詳細を見る
A thermostable mediator-type enzyme glucose sensor was constructed. The electrode was fabricated using chemically cross-linked thermostable co-factor binding glucose dehydrogenase (GDH) from thermophilic bacteria in carbon paste matrix. The electrode responded directly proportional to D-glucose concentration from 0.01 mM to 3 mM in stirred buffer containing 1 mM 1-methoxyphenazinemethosulfate as a mediator with the steady-state mode. The storage stability was examined by incubating the enzyme electrode at 50oC during the measurement. The cross-linked GDH immobilized electrode showed good storage stability. Ninety percent of its initial response was retained after incubation in buffer solution for 9 days at 50oC. The flow injection analysis (FIA) glucose sensing system was also constructed by immobilizing the cross-linked GDH and ferrocene as a mediator in the carbon paste matrix. The FIA system was able to measure 600 samples for 100 h.
- 社団法人 電気学会の論文
- 2003-06-01
著者
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SODE Koji
Department of Biotechnology, Tokyo University of Agriculture and Technology
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Yamazaki Tomohiko
Dept.biotechnolgy & Life Science Tokyo University Of Agriculture And Technology.
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NAKAZAWA Yukie
Dept.Biotechnolgy & Life science, Tokyo University of Agriculture and technology.
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TSUGAWA Wakako
Dept.Biotechnolgy & Life science, Tokyo University of Agriculture and technology.
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IKEBUKURO Kazunori
Dept.Biotechnolgy & Life science, Tokyo University of Agriculture and technology.
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SODE Koji
Dept.Biotechnolgy & Life science, Tokyo University of Agriculture and technology.
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Tsugawa W
Tokyo Univ. Agriculture And Technol. Tokyo
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Tsugawa Wakako
Department Of Biotechnology Tokyo University Of Agriculture And Technology
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Tsugawa Wakako
Dept.biotechnolgy & Life Science Tokyo University Of Agriculture And Technology.
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Nakazawa Yukie
Dept.biotechnolgy & Life Science Tokyo University Of Agriculture And Technology.
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Ikebukuro Kazunori
Dept.biotechnolgy & Life Science Tokyo University Of Agriculture And Technology.
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Sode Koji
Dept.biotechnolgy & Life Science Tokyo University Of Agriculture And Technology.
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Sode Koji
Dept. Biotechnol. Tokyo Univ. Agric. Technol.
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Sode K
Dept.biotechnolgy & Life Science Tokyo University Of Agriculture And Technology.
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Ikebukuro Kazunori
Dept. Biotechnolgy & Life science, Tokyo University of Agriculture and technology
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Nakazawa Yukie
Dept. Biotechnolgy & Life science, Tokyo University of Agriculture and technology
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Tsugawa Wakako
Dept. Biotechnolgy & Life science, Tokyo University of Agriculture and technology
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Yamazaki Tomohiko
Dept. Biotechnolgy & Life science, Tokyo University of Agriculture and technology
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