Predominant Growth of α-Amylase Regulation Mutant in Continuous Culture of Bacillus caldolyticus
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概要
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The production of a-amylase in batch and continuous cultures of the strain SP of Bacillus caldolyticus was studied using a maltose-castione medium. The three quantitative parameters of a-amylase production (maximum values of the specific production rate, volumetric productivity and concentration of α-amylase) increased in continuous culture by 2.5,4.6 and 3.8 times respectively in comparison with a batch culture. It was found that a mutant strain M1 of α-amylase production was predominant in every run of the continuous cultures. The strain M1 differs from the strain SP in that it can produce α-amylase constitutively in a batch culture while the cells of strain SP require maltose for α-amylase production. On the contrary, α-amylase production by the strain M1 was repressed partly by maltose. Moreover, glucose repression on α-amylase production was not observed for the M1 strain while it was remarkable for the strain SP. The above-mentioned properties of the mutant M1 concerning the regulation of a α-amylase production are not only advantageous for industrial use but also interesting from the viewpoint of basic microbiology.
- 1989-03-25
著者
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Toda Kiyoshi
Institute Of Applied Microbiology University Of Tokyo
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YABE ISAMU
Institute of Molecular and Cellular Biosciences, University of Tokyo
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Cheng Chu
Institute Of Applied Microbiology University Of Tokyo
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Toda Kiyoshi
Institute Of Applied Microbiology The University Of Tokyo
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Yabe Isamu
Institute Of Applied Microbiology University Of Tokyo
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