A kinetic analysis of proton transport in bacteriorhodopsin-liposomes based on bilateral pH determinations.
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概要
- 論文の詳細を見る
Simultaneous changes of pH in the inner and outer liquids of proteoliposomes, the membrane of which consisted of asolectin and bacteriorhodopsin (BR), were determined during transient states after a shift from the light to the dark and the reverse. Some mathematical equations of a model characterizing the mechanism of the proton transport through the proteoliposome membrane were developed by considering the proton pump of BR, lightdependent and light-independent proton leaks from the liposomes, and buffering capacities of the intra- and extravesicular liquids. The model equations (equations 8(A) and 8(B)) could assess the effect of concurrent change of the bilateral proton concentrations on the rate of proton transport and could simulate well the dynamic aspect of pH changes within the internal and external liquid of the proteoliposomes.
- 公益社団法人 化学工学会の論文
著者
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TODA KIYOSHI
Institute of Applied Microbiology, The University of Tokyo
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Toda Kiyoshi
Institute Of Applied Microbiology The University Of Tokyo
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Yabe Isamu
Institute Of Applied Microbiology University Of Tokyo
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Matsuoka Hiroshi
Department Of Applied Chemistry Faculty Of Engineering Osaka University
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YABE ISAMU
Institute of Applied Microbiology, The University of Tokyo
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