Detection of Protease Activity with a Fluorescence-labelled Peptide Substrate on a TLC Plate
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概要
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A small amount of peptidase activity could be detected using an amine derivatizing reagent, fluorescein isothiocyanate (FITC), which has been used to produce a fluorogenic peptide. The substrate produced, FITC-peptide, gave a clear spot on a silica gel sheet upon exposure to UV LIGHT. The peptidase activity of angiotensin-converting enzyme (ACE), trypsin, chymotrypsin, cucumisin, and that of some plant tissues were detected by using a fluorogenic angiotensin I. This showed that the substrate specificity of proteolytic enzyme can be distinguished form the others by this procedure.
- 社団法人日本農芸化学会の論文
- 2000-06-23
著者
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KANEDA MAKOTO
Department of Pediatrics, Hokkaido University Graduate School of Medicine
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Kaneda M
Department Of Chemistry Faculty Of Science Kagoshima University
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YONEZAWA Hiroo
Department of Chemistry, Faculty of Science, Kagoshima University
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UCHIKOBA Tetsuya
Department of Chemistry, Faculty of Science, Kagoshima University
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ARIMA Kazunari
Plant Gene Expression Center, Department of Plant Biology, University of California
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YONEZAWA Hiroo
Laboratory of Biochemistry, Department of Chemistry, Faculty of Science, Kagoshima University
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Kaneda Makoto
Department Of Chemistry Faculty Of Science Kagoshima University
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Yonezawa Hiroo
Laboratory Of Biochemistry Department Of Chemistry Faculty Of Science Kagoshima University
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Arima Kazunari
Laboratory Of Biochemistry Department Of Chemistry Faculty Of Science Kagoshima University
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UCHIKOBA Tetsuya
Kagoshima University Musium
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Uchikoba T
Kagoshima University Musium
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Uchikoba Tetsuya
Department Of Chemistry Faculty Of Science Kagoshima University
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