Evidence for Structural Differences between the Two Highly Homologous Actin-regulatory Proteins, Destrin and Cofilin

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概要

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• The amino acid sequences of destrin and cofilin are very similar (84% homology) throughout the entire range of proteins, but they have different functions. In this study, we constructed a new cofilin expression plasmid, which had high expression frequency, and the structures of destrin and cofilin were analyzed by limited proteolysis and circular dichroism (CD). When destrin was digested by trypsin, two fragments of 17.0kDa and 9.2kDa were obtained, whereas only one 8.4kDa fragment was obtained from cofilin. In spite of the overall sequence homology, an N-terminal amino acid sequence analyses of the fragments revealed the cleavage sites on destrin and cofilin to be different. These results suggest that destrin and cofilin differ in their overall tertiary folds. Cofilin showed activity similar to destrin at high pH values, although no pH-dependent structural change in cofilin was confirmed by using limited proteolysis and CD.

• 社団法人日本農芸化学会の論文
• 1998-02-23

著者

• ARIMA Kazunari

  Department of Plant Biology, Plant Gene Expression Center

• Kazuta Yasuaki

  Department of Bioinformatic Engineering, Osaka Univeristy

• Kazuta Yasuaki

  Department Of Biochemical Engineering And Science Faculty Of Computer Science And Systems Engineerin

• IMANAKA Minako

  Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineeri

• OKUZONO Seigou

  Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineeri

• KOTANI Susumu

  Department of Biochemical Engineering and Science, Faculty of Computer Science and Systems Engineeri

• Kotani Susumu

  Department Of Biochemical Engineering And Science Faculty Of Computer Science And Systems Engineerin

• Imanaka M

  Department Of Biochemical Engineering And Science Faculty Of Computer Science And Systems Engineerin

• Okuzono Seigou

  Department Of Biochemical Engineering And Science Faculty Of Computer Science And Systems Engineerin

• Arima Kazunari

  Laboratory Of Biochemistry Department Of Chemistry Faculty Of Science Kagoshima University

• Arima Kazunari

  Department Of Chemistry And Bioscience Faculty Of Science Kagoshima University

• Imanaka Minako

  Department Of Biochemical Engineering And Science Faculty Of Computer Science And Systems Engineerin

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