Detection of Hydrolytic Activity of Trypsin with a Fluorescence-chymotryptic Peptide on a TLC Plate
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概要
- 論文の詳細を見る
To find a new trypsin-like enzyme, a simple assay method of the hydrolysis activity for trypsin has been found. We used 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) in the peptide labeling as a substrate for the trypsin-like peptidase in this study. The peptidase activity of trypsin was detected by using an AQC-chymotryptic peptide (AHP1) obtained from bovine hemoglobin. This showed that the substrate specificity of trypsin-like peptidase was distinguishable from that of the others by this procedure, and the method was used extensively in cases of various trypsin inhibitors with no significant interference from the concomitant.
- 社団法人 日本農芸化学会の論文
- 2004-01-23
著者
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YONEZAWA Hiroo
Laboratory of Biochemistry, Department of Chemistry, Faculty of Science, Kagoshima University
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Itakura Takao
Department Of Biochemistry Faculty Of Veterinary Medicine Hokkaido University
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Itakura Takao
Department Of Biochemistry And Technology Of Aquatic Resources Faculty Of Fisheries Kagoshima Univer
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Yonezawa Hiroo
Laboratory Of Biochemistry Department Of Chemistry Faculty Of Science Kagoshima University
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Arima Kazunari
Laboratory Of Biochemistry Department Of Chemistry Faculty Of Science Kagoshima University
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Okubo Michiko
Department Of Health And Nutrition Kagoshima Immaculate Heart University
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UCHIKOBA Tetsuya
Kagoshima University Musium
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FUKUMOTO Shigeko
Kagoshima University Musium
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TOMOKIYO Kazuhiko
The Chemo-Sero-Therapeutic Research Institute
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Uchikoba T
Kagoshima University Musium
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