Fluorogenic Substrates for Cathepsin D

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概要

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• Fluorogenic substrates for cathepsin D; A-Tyr-Phe (NO_2)-Leu-Leu (A; Ala-Arg-Pro-Lys-Pro-Leu-Leu-, Arg-Pro-Lys-Pro-Leu-Leu-, Pro-Lys-Pro-Leu-Leu-, Lys-Pro-Leu-Leu-, Pro-Leu-Leu-) and B-Phe (NO_2)-Tyr-Leu-Leu(B; Arg-Pro-Lys-Pro-Leu-Leu-, Pro-Lys-Pro-Leu-Leu-, Lys-Pro-Leu-Leu-, Pro-Leu-Leu-) (Phe(NO_2), p-nitrophenylalanine) were synthesized and digested by cathepsin D and pepsin. The fluorescence at 303nm (excitation at 260nm) was increased with the hydrolysis of the substrates. The minimum detectable cathepsin D concentrations for these substrates were 0.5-4nM and pepsin concentrations were 0.1-0.8nM except Pro-Leu-Leu-Tyr-Phe(NO_2)-Leu-Leu under the following conditions : substrate concentration, 20μM; measuring time, 3min. The hydrolysis rate constants (kcat/Km) of B-Phe(NO_2)-Tyr-Leu-Leu for cathepsin D were same or 2-3 times greater than A-Tyr-Phe(NO_2)-Leu-Leu. On the other hand, those of B-Phe(NO_2)-Tyr-Leu-Leu for pepsin were the same or 4-20 times greater than A-Tyr-Phe(NO_2)-Leu-Leu. The hydrolysis rates of the substrates by both enzymes tend to increase with the increase of the peptide chain length. The best substrate for cathepsin D was Arg-Pro-Lys-Pro-Leu-Leu-Phe(NO_2)-Tyr-Leu-Leu and its kcat/Km was 1.3μM^<-1>s^<-1>.

• 社団法人日本農芸化学会の論文
• 1999-08-23

著者

• KANEDA MAKOTO

  Department of Pediatrics, Hokkaido University Graduate School of Medicine

• Kaneda M

  Department Of Chemistry Faculty Of Science Kagoshima University

• UCHIKOBA Tetsuya

  Department of Chemistry, Faculty of Science, Kagoshima University

• ARIMA Kazunari

  Department of Plant Biology, Plant Gene Expression Center

• YONEZAWA Hiroo

  Laboratory of Biochemistry, Department of Chemistry, Faculty of Science, Kagoshima University

• Kaneda Makoto

  Department Of Chemistry Faculty Of Science Kagoshima University

• Yonezawa Hiroo

  Laboratory Of Biochemistry Department Of Chemistry Faculty Of Science Kagoshima University

• Arima Kazunari

  Laboratory Of Biochemistry Department Of Chemistry Faculty Of Science Kagoshima University

• Arima Kazunari

  Department Of Chemistry And Bioscience Faculty Of Science Kagoshima University

• UCHIKOBA Tetsuya

  Kagoshima University Musium

• Uchikoba T

  Kagoshima University Musium

• Uchikoba Tetsuya

  Department Of Chemistry Faculty Of Science Kagoshima University

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