Substrate Specificity of Cucumisin on Synthetic Peptides

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概要

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• The substrate specificity of cucumisin [EC 3.4.21.25] was identified by the use of the synthetic peptide substrates Leu_m-Pro-Glu-Ala-Leu_n (m=0-4,n=0-3). Neither Pro-Glu-Ala-Leu (m=0) nor Leu-Pro-Glu-Ala (n=0) was cleaved by cucumisin, however other analogus peptides were cleaved between Glu-Ala. The hydrolysis rates of Leu_m-Pro-Glu-Ala-Leu increased with the increase of m=1 to 2 and 3,but was however, essentially same with the increase of m=3 to 4. Similarly, the hydrolysis rates of Leu-Leu-Pro-Glu-Ala-Leu_n increased with the increase of n=0 to 1 and 2,but was essentially same with the increase of n=2 to 3. Then, it was concluded that cucumisin has a S_5-S'_3 subsite length. In order to identify the substrate specificity at P_1 position, Leu-Leu-Pro-X-Ala-Leu (X ; Gly, Ala, Val, Leu, Ile, Pro, Asp, Glu, Lys, Arg, Asn, Gln, Phe, Tyr, Ser, Thr, Met, Trp, His) were synthesized and digested by cucumisin. Cucumisin showed broad specificity at the P_1 position. However, cucumisin did not cleave the C-terminal side of Gly, Ile, Pro, and preferred Leu, Asn, Gln, Thr, and Met, especially Met. Moreover, the substrates, Leu-Leu-Pro-Glu-Y-Leu (Y ; Gly, Ala, Ser, Leu, Val, Glu, Lys, Phe) were synthesized and digested by cucumisin. Cucumisin did not cleave the N-terminal side of Val but preferred Gly, Ser, Ala, and Lys especially Ser. The specificity of cucumisin for naturally occurring peptides does not agree strictly with the specificity obtained by synthetic peptides at the P_1 or P'_1 position alone, but it becomes clear that the most of the cleavage sites on naturally occurring peptides by cucumisin contain suitable amino acid residues at P_1 and (or) P'_1 positions. Moreover, cucumisin prefers Pro than Leu at P_2 position, indicating that the specificity at P_2 position differs from that of papain.

• 社団法人日本農芸化学会の論文
• 2000-10-23

著者

• KANEDA MAKOTO

  Department of Pediatrics, Hokkaido University Graduate School of Medicine

• Kaneda M

  Department Of Chemistry Faculty Of Science Kagoshima University

• Kaizuka Hideki

  Department Of Chemistry Faculty Of Science Kagoshima University

• YONEZAWA Hiroo

  Department of Chemistry, Faculty of Science, Kagoshima University

• UCHIKOBA Tetsuya

  Department of Chemistry, Faculty of Science, Kagoshima University

• ARIMA Kazunari

  Plant Gene Expression Center, Department of Plant Biology, University of California

• YONEZAWA Hiroo

  Laboratory of Biochemistry, Department of Chemistry, Faculty of Science, Kagoshima University

• Kaneda Makoto

  Department Of Chemistry Faculty Of Science Kagoshima University

• Yonezawa Hiroo

  Laboratory Of Biochemistry Department Of Chemistry Faculty Of Science Kagoshima University

• Arima Kazunari

  Laboratory Of Biochemistry Department Of Chemistry Faculty Of Science Kagoshima University

• UCHIKOBA Tetsuya

  Kagoshima University Musium

• Uchikoba T

  Kagoshima University Musium

• Uchikoba Tetsuya

  Department Of Chemistry Faculty Of Science Kagoshima University

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