Purification and Some Properties of a Protease from the Sarcocarp of Musk Melon Fruit
スポンサーリンク
概要
- 論文の詳細を見る
A protease has been purified from sarcocarp of musk melon, Cucumis melo ssp. melo var. reticulatus Naud. Earl's Favourite. The protease was mostly present in the placenta part of the fruit and next in the inside mesocarp. The molecular mass of the enzyme was estimated to be about 62 kDa on SDS-PAGE. The enzyme had a carbohydrate moiety. The optimum pH of the enzyme was 11 at 35℃ using casein as a substrate. The enzyme was stable between pH 6 and 11. The enzyme was strongly inhibited by diisopropyl fluorophosphate, but was not inhibited by EDTA or cysteine protease inhibitors. From the digestion of Ala-Ala-Pro-X-pNA (X = Phe, Leu, Val, Ala, Gly, Lys, Glu, Pro, and diaminopropionic acid (Dap) substrates the specificity of the protease was found to be approximately broad, but the preferential cleavage sites were C-terminal sites of hydrophobic or acidic amino acid residues at P_1 position. It was proved that the enzymatic properties of musk melon protease are similar to those of cucumisin [EC 3.4.21.25]. The enzyme was not inhibited by typical proteinous inhibitors such as STI or ovomucoid. Therefore, this enzyme seems to be a useful protease for the food industries.
- 社団法人日本農芸化学会の論文
- 1997-12-23
著者
-
KANEDA MAKOTO
Department of Pediatrics, Hokkaido University Graduate School of Medicine
-
Kaneda M
Department Of Chemistry Faculty Of Science Kagoshima University
-
YONEZAWA Hiroo
Department of Chemistry, Faculty of Science, Kagoshima University
-
UCHIKOBA Tetsuya
Department of Chemistry, Faculty of Science, Kagoshima University
-
YONEZAWA Hiroo
Laboratory of Biochemistry, Department of Chemistry, Faculty of Science, Kagoshima University
-
Kaneda Makoto
Department Of Chemistry Faculty Of Science Kagoshima University
-
Yonezawa Hiroo
Laboratory Of Biochemistry Department Of Chemistry Faculty Of Science Kagoshima University
-
UCHIKOBA Tetsuya
Kagoshima University Musium
-
Uchikoba T
Kagoshima University Musium
-
Uchikoba Tetsuya
Department Of Chemistry Faculty Of Science Kagoshima University
関連論文
- 原発性免疫不全症に対する造血幹細胞移植
- Substrate Specificity of Cucumisin on Synthetic Peptides
- Detection of Protease Activity with a Fluorescence-labelled Peptide Substrate on a TLC Plate
- Isolation and Characterization of Pepsinogen from Trimeresurus flavoviridis (Habu Snake)
- Isolation and Some Properties of a Serine Protease from the Fruits of Cudrania cochinchinensis (Lour.) Kudo et Masam.
- Fluorogenic Substrates for Cathepsin D
- Amino Acid Sequence and Some Properties of Phytolacain R, a Cysteine Protease from Full-Growth Fruits of Pokeweed, Phytolacca americana
- Comparison of Phytolacain G, a Cysteine Protease from Fruit of Phytolacca americana, with Phytolacain R
- A Cysteine Protease from Young Stems of Asparagus : Isolation, Properties, and Substrate Specificity
- Purification and Some Properties of a Protease from the Sarcocarp of Musk Melon Fruit
- Purification and Characterization of a Cysteine Protease from Corms of Freesia, Freesia reflacta
- Substrate Specificity of Honeydew Melon Protease D, a Plant Serine Endopeptidase
- Determination of Pepstatin-Sevnsitive Carboxyl Proteases by Using PePstatinyldansyldiaminopropane (Dansyl-Pepstatin) as an Active Site Titrant
- Identificationof the Reactive Histidine of Cucumisin, a Plant Serine Protease: Modification with Peptidyl Chloromethyl Ketone Derivative of Peptide Substrate
- Isolation of Phytolacain G and Its Inhibition Measured by Affinity Labeling
- Cleavage Specificity of Cucumishi, a Plant Serine Protease
- Susceptibility-Weighted Magnetic Resonance Imaging Detects Impaired Cerebral Hemodynamics in the Superior Sagittal Sinus Thrombosis : Case Report
- Prolonged Bone Marrow Failure With Monosomy 7 After Engraftment Failure Following Bone Marrow Transplantation
- Amino Acid Sequences of Glycopeptides from Cucumisin
- Detection of Hydrolytic Activity of Trypsin with a Fluorescence-chymotryptic Peptide on a TLC Plate
- Isolation and Characterization of a Cysteine Protease of Freesia Corms(Biochemistry & Molecular Biology)
- Purification and Some Properties of an Aminopeptidase from the Seeds of Cannabis sativa
- Photochemical Oxidation of Cucumisin, a Serine Proteinase from Cucumis melo(Biological Chemistry)
- Properties of a New Plant Serine Protease Cucumisin(Biological Chemistry)
- Development of germinoma during the treatment of systemic-onset juvenile idiopathic arthritis with infliximab
- Specificity of Cucumisin in Hydrolysis of Peptide Thiobenzyl Esters
- Photoaffinity labeling of pepsin.
- Epidemiology and treatment outcome of invasive fungal infections in patients with hematological malignancies