Glutamate Overproduction in Corynebacterium glutamicum Triggered by a Decrease in the Level of a Complex Comprising DtsR and a Biotin-containing Subunit

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概要

• 論文の詳細を見る

• Glutamate overproduction in Corynebacterium glutamaicum is induced by Tween 40,biotin-limitation, or sublethal amounts of penicillin. Disruption of the dtsR gene, which encodes a putative component of a biotin-containing enzyme complex involved in fatty acid synthesis, causes constitutive overproduction of glutamate. We report here that overexpression of dtsR inhibits the induction of glutamate overproducation. In contrast, the level of DtsR in the wild type strain was found to decrease in the presence of Tween 40 or limited amounts of biotin. Tween 40,biotinlimitation, or dtsR disruption also reduced the activity of 2-oxoglutarate dehydrogenase complex (ODHC), which is involved in the synthesis of succinate from 2-oxoglutarate. These results indicate that decrease in the level of DtsR or a complex containing DtsR triggers the increased synthesis of glutamate from 2-oxoglutarate by lowering the ODHC activity.

• 社団法人日本農芸化学会の論文
• 1999-07-23

著者

• Tokuda H

  Univ. Tokyo Tokyo

• KIMURA Eiichiro

  Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.

• YAGOSHI Chizu

  Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.

• KAWAHARA Yoshio

  Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.

• OHSUMI Tsuyoshi

  Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.

• NAKAMATSU Tsuyoshi

  Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.

• TOKUDA Hajime

  Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.

• Tokuda Hajime

  Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.

• Yagoshi Chizu

  Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.

• Ohsumi Tsuyoshi

  Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.

• Kawahara Y

  Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.

• Kawahara Yoshio

  Fermentation & Biotechnology Laboratories Ajinomoto Co. Inc.

• Nakamatsu T

  Biological Institute Faculty Of Education Yamaguchi University

• Kimura Eiichiro

  R & D Management Department Ajinomoto Co. Inc.

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• Glutamate Overproduction in Corynebacterium glutamicum Triggered by a Decrease in the Level of a Complex Comprising DtsR and a Biotin-containing Subunit
• The Energetic Conversion Competence of Escherichia coli during Aerobic Respiration Studied by ^P NMR Using a Circulating Fermentation System
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• Simultaneous and High Fermentative Production of L-Lysine and L-Glutamic Acid Using a Strain of Brevibacterium lactofermentum
• Molecular Cloning of a Novel Gene, dtsR, Which Rescues the Detergent Sensitivity of a Mutant Derived from Brevibacterium lactofermentum
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