Molecular Cloning of a Novel Gene, dtsR, Which Rescues the Detergent Sensitivity of a Mutant Derived from Brevibacterium lactofermentum

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概要

• 論文の詳細を見る

• Several strains of Corynebacterium and Brevibacterium are known for their ability to secrete large amounts of amino acids, especially L-glutamate. We focused on the mechanism of L-glutamate secretion triggered by a detergent, namely polyoxyethylenesorbitan monopalmitate (PESP). A mutant strain, AJ11060, derived from Brevibacterium lactofermentum ATCC 13869 indicates the sensitivity to PESP. A multicopy suppresser gene that compliments the sensitivity of AJ11060 to the detergent was derived from a gene library of B. lactofermentum AJ12036. A 2855-bp DNA fragment was cloned and sequenced. An open reading frame was found that coded for the rescuer gene of the sensitivity to PESP of AJ11060 and was designated dtsR. The expression of the dtsR gene in B. lactofermentum was confirmed by using anti-DtsR antibody. The deduced DtsR protein indicated significant homology with some biotin enzymes such as the β chain of propionyl-CoA carboxylase from rat (48.3%) and human (48.7%), or a 12S chain of methylmalonyl-CoA carboxyltransferase from Propionibacterium freudenreichii (43.1%).

• 社団法人日本農芸化学会の論文
• 1996-10-23

著者

• KAWAHARA Yoshio

  Fermentation and Biotechnology Laboratories, Ajinomoto Co., Inc.

• Kawahara Y

  Fermentation And Biotechnology Laboratories Ajinomoto Co. Inc.

• Kawahara Yoshio

  Technology And Engineering Center Of Ajinomoto Co. Inc.

• Nakamatsu T

  Biological Institute Faculty Of Education Yamaguchi University

• Nakamatsu Tsuyoshi

  Technology And Engineering Laboratories Of Ajinomoto Co. Inc.

• KIMURA Eiichiro

  Technology and Engineering Laboratories, Ajinomoto Co., Inc.

• ABE Chizu

  Technology and Engineering Laboratories, Ajinomoto Co., Inc.

• Kimura Eiichiro

  R & D Management Department Ajinomoto Co. Inc.

• Abe Chizu

  Technology And Engineering Laboratories Ajinomoto Co. Inc.

関連論文

• Glutamate Overproduction in Corynebacterium glutamicum Triggered by a Decrease in the Level of a Complex Comprising DtsR and a Biotin-containing Subunit
• The Energetic Conversion Competence of Escherichia coli during Aerobic Respiration Studied by ^P NMR Using a Circulating Fermentation System
• Proline in the Osmoregulation of Brevibacterium lactofermentum(Biological Chemistry)
• Relationship between the Glutamate Production and the Activity of 2-Oxoglutarate Dehydrogenase in Brevibacterium lactofermentum
• Purification and Characterization of Malate Dehydrogenase from Corynebacterium glutamicum(ENZYMOLOGY,PROTEIN ENGINEERING,AND ENZYME TECHNOLOGY)
• Simultaneous and High Fermentative Production of L-Lysine and L-Glutamic Acid Using a Strain of Brevibacterium lactofermentum
• Molecular Cloning of a Novel Gene, dtsR, Which Rescues the Detergent Sensitivity of a Mutant Derived from Brevibacterium lactofermentum
• Triggering Mechanism of L-Glutamate Overproduction by Dts1 in Coryneform Bacteria(BREWING AND FOOD TECHNOLOGY)

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