Triggering Mechanism of L-Glutamate Overproduction by Dts1 in Coryneform Bacteria(BREWING AND FOOD TECHNOLOGY)

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概要

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• The mechanism of L-glutamate-overproduction by Corynebacterium glutamicum, a biotin auxotroph, is very unique and interesting. L-Glutamate overproduction by this bacterium is induced by biotin-limitation and suppressed by an excess of biotin. Addition of a surfactant or penicillin is also induces L-glutamate overproduction even under excess biotin. After the development of general molecular biological tools such as cloning vectors and DNA transfer techniques, genes encoding biosynthetic enzymes were isolated. With those genes and tools, recombinant DNA technology can be applied to the analysis of biosynthetic pathways and the construction of C. glutamicum strains. In this review, recent studies on the triggering mechanism of L-glutamate overproduction by C. glutamicum are discussed. Disruption of the dtsR1 gene, which encodes a putative component of a biotin-containing enzyme complex that is involved in fatty acid synthesis, causes constitutive overproduction of L-glutamate. As in the case of biotin-limitation, i.e., addition of a surfactant or penicillin, dtsR1-disruption also reduces the activity of the 2-oxoglutarate dehydrogense complex (ODHC). These results indicate that the DtsR1 level affects the activity of ODHC. In our recent studies, a novel regulatory factor that suppresses the expression of DtsR1 was determined. Based on these findings, the triggering mechanism of L-glutamate overproduction is expected to be clarified in more detail.

• 公益社団法人日本生物工学会の論文
• 2002-12-25

著者

• Kimura Eiichiro

  R & D Management Department Ajinomoto Co. Inc.

• KIMURA EIICHIRO

  R & D Management Department, Ajinomoto Co., Inc.

関連論文

• Glutamate Overproduction in Corynebacterium glutamicum Triggered by a Decrease in the Level of a Complex Comprising DtsR and a Biotin-containing Subunit
• Molecular Cloning of a Novel Gene, dtsR, Which Rescues the Detergent Sensitivity of a Mutant Derived from Brevibacterium lactofermentum
• Triggering Mechanism of L-Glutamate Overproduction by Dts1 in Coryneform Bacteria(BREWING AND FOOD TECHNOLOGY)

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