Purification of Three Acidic Chitinases from Yam Aerial Tuber. Application of Quaternary Ammonium Ion Detergent for the Separation of Yam Chitinase from Viscous Tissue Extract
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概要
- 論文の詳細を見る
Three inherent acidic chitinases were puritied from yam aerial tuber by the application of quaternary ammonium ion detergent. For removal of the mucilage in the crude extract of aerial tuber, the effects of various quaternary ammonium ion detergent were compared. Cetylpyridinium chloride effectively removed the mucilage and this method proved to provide high recovery of chitinase activity. Three acidic chitinase isoforms were purified by anion-exchange chromatography, gel-filtration chromatography, and anion-exchange HPLC from the supernatant of cetylpyridinium chloride treatment. The three purified acidic chitinases had the molecular masses of 23, 25, and 31kDa and proved to be class III, class III, and class I chitinases, respectively, by the comparison of tryptic peptide maps and amino acid compositions.
- 社団法人日本農芸化学会の論文
- 1995-03-23
著者
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ARAKI Tomohiro
Department of Bioscience, School of Agriculture, Tokai University
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Araki T
Department Of Bioscience School Of Agriculture Kyushu Tokai University
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Araki Tomohiro
Department Of Bioscience School Of Agriculture Kyushu Tokai University
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Torikata T
Department Of Bioscience School Of Agriculture Tokai University
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Torikata Takao
Laboratory Of Sericultural Chemistry Faculty Of Agriculture Kyushu University
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ARAKI Tomohiro
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu Tokai University
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Kuramoto Mayumi
Laboratory of Biochemistry, School of Agriculture, Kyushu Tokai University
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Kuramoto Mayumi
Laboratory Of Biochemistry School Of Agriculture Kyushu Tokai University
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