Amino Acid Sequence of Egyptian Goose Egg-White Lysozyme and Effects of Amino Acid Substitution on the Enzymatic Activity
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概要
- 論文の詳細を見る
The amino acid sequence of Egyptian goose lysozyme (EGL) from egg-white and its enzymatic properties were analyzed. The established sequence had the highest similarity to wood duck lysozyme (WDL) with five amino acid substitutions, and had eighteen substitutions difference from hen egg-white lysozyme (HEL). Tyr34 and Gly37 were found at subsites E and F of the active site when compared with HEL. The experimental time-course characteristics of EGL against the <I>N</I>-acetylglucosamine pentamer substrate, (GlcNAc)<SUB>5</SUB>, revealed higher production of (GlcNAc)<SUB>4</SUB> and lower production of (GlcNAc)<SUB>2</SUB> when compared with HEL. The saccharide-binding ability of subsites A–C in EGL was also found to be weaker than in HEL. An analysis of the enzymatic reactions of five mutants in respect of positions 34, 37 and 71 in HEL indicated the time-course characteristics of EGL to be caused by the combination of three substitutions (F34Y, N37G and G71R) between HEL and EGL. A computer simulation of the EGL-catalyzed reaction suggested that the time-course characteristics of EGL resulted from the difference in the binding free energy for subsites A, B, E and F and the rate constant of transglycosylation between EGL and <I>HEL</I>.
- Japan Society for Bioscience, Biotechnology, and Agrochemistryの論文
- 2012-04-23
著者
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Kawamura Shunsuke
Department Of Bioscience School Of Agriculture Tokai University
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ARAKI Tomohiro
Department of Bioscience, School of Agriculture, Tokai University
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Kuhara Satoru
Faculty Of Agriculture Kyushu University
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Chijiiwa Yuki
Department of Bioscience, School of Agriculture, Tokai University
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Toshima Gen
Department Of Bioscience School Of Agriculture Kyushu Tokai University
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Araki Tomohiro
Department Of Biochemistry School Of Agriculture Kyushu Tokai University
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Torikata T
Department Of Bioscience School Of Agriculture Tokai University
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Torikata Takao
Department Of Biochemistry School Of Agriculture Kyushu Tokai University
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Satou Kenji
Human Genome Center Institute Of Medical Science The University Of Tokyo
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Kuhara S
Department Of Bioscience School Of Agriculture Tokai University
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Chijiiwa Yuki
Department Of Bioscience School Of Agriculture Tokai University
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