抗リンパ球血清に関する研究 : 第2編.作用機序に関する実験的研究

概要

論文の詳細を見る
The mode of action of the anti-lymphocyte rabbit serum (ALS) was studied by the fluorescent antibody technique. In vivo tissue localization of ALS was observed by fluorescent microscope after the single injection of ALS conjugated with FITC. In vitro reactivity of the conjugated anti-serum with the frozen sections or impregnant preparations of mouse lymphoid tissues were also studied. After intravenous injection into mice, ALS accumulated mainly in the lymphoid organs. Deposition of the conjugated ALS which probably represented antigen-antibldy complex was observed in the glomeruli after repeated injection of ALS. In vitro preparations, the conjugated anti-serum stained lymphocytes specifically. Plasma cells proliferated in the lymphoid tissue of the mice received the repeated injection of ALS showed cytoplasmic fluorescence that was indicative of the production of the antibody against ALS. These findings suggested that the immunological unresponsiveness of the mouse injected with ALS was due to the block of the biological activity of lymphoid cells as a result of the antigen-antibody reaction. Following these experimental data, author proposed the new hypotheses of "partial immune tolerance theory" as the mode of action of ALS.
日本アレルギー学会の論文
1969-09-30