Delayed Expression of Apoptosis in X-irradiated Human Leukemic MOLT-4 Cells Transfected with Mutant p53
スポンサーリンク
概要
- 論文の詳細を見る
The effects of X-rays on cell survival, apoptosis, and long-term response in the development of cell death as measured by the dye exclusion test were studied in human leukemic MOLT-4 cells (p53 wild-type) stably transfected with a mutant p53 cDNA expression vector. Cell survival, as determined from colony-forming ability, was increased in an expression level dependent manner, but the increase was partial even with the highest-expressing clone (B3). This contrasts with the prior observation that cell death and apoptosis in B3 are completely inhibited at 24 h after irradiation with 1.8 Gy of X-rays. The examination of B3 cells incubated for longer than 24 h after X-irradiation showed a delay in the induction of cell death and apoptosis. Western blot analysis revealed that the time required to reach the highest level of wild-type p53 protein in B3 was longer than the time in MOLT-4 and that the p53 may be stabilized by the phosphorylation at Ser-15. These results suggest that the introduction of mutant p53 into MOLT-4 merely delays the development of apoptosis, during which the cells could repair the damage induced by X-rays, and results in the partial increase in cell survival.
- 日本放射線影響学会の論文
著者
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YONEKAWA Hiromichi
Department of Laboratory Animal Science, The Tokyo Metropolitan Institute of Medical Science
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Yonekawa Hiromichi
Department Of Laboratory For Animal Science Tokyo Metropolitan Institute Of Medical Science
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Yonekawa Hiromichi
Tokyo Metropolitan Inst. Of Medical Sci.
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Yonekawa Hiromichi
Dep. Of Lab. Animal Sci. The Tokyo Metropolitan Inst. Of Medical Sci.
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Yonekawa Hiromichi
Department Of Laboratory Animal Science The Tokyo Metropolitan Institute Of Medical Science (rinshok
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NAKANO HISAKO
Department of Laboratory Animal Science, Tokyo Metropolitan Institute of Medical Science
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SHINOHARA KUNIO
Radiation Research Institute, Graduate School of Medicine, The University of Tokyo
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Nakano Hisako
東京大学医学部放射線研究施設
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Nakano Hisako
Department Of Laboratory Animal Science Tokyo Metropolitan Institute Of Medical Science
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Nakano Hisako
Dept. Radiat. Res. Tokyo Metropolitan Inst. Med. Sci.
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Shinohara Kunio
東京大学医学部放射線研究施設
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Shinohara Kunio
Advanced Research Institute For Science And Engineering Waseda University
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Yonekawa H
Department Of Laboratory Animal Science The Tokyo Metroporitan Institute Of Medical Science
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Nakano Hisako
Department Of Food Safety Tokyo Metropolitan Institute Of Public Health
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Shinohara K
Radiation Research Institute Graduate School Of Medicine The University Of Tokyo
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Shinohara K
Hokkaido Univ. Sapporo‐shi
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Yonekawa H
Tokyo Metropolitan Inst. Of Medical Sci.
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Yonekawa Hiromichi
Department Of Animal Science The Tokyo Metropolitan Institute Of Medical Science
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Shinohara Kunio
Radiation Research Institute Graduate School Of Medicine The University Of Tokyo
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