Role of Wild-type p53 in Apoptotic and Non-Apoptotic Cell Death Induced by X-irradiation and Heat Treatment in p53-mutated Mouse M10 Cells

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概要

• 論文の詳細を見る

• The sensitizing effects of wild-type p53 on X-ray-induced cell death and on heat-induced apoptosis in M10, a radiosensitive and Trp53 (mouse p53 gene)-mutated lymphoma cell line which dies through necrosis by X-irradiation, were investigated using three M10 derived transfectants with wild-type TP53 (human p53 gene). Cell death was determined by colony formation and/or dye exclusion test, and apoptosis was detected as the changes in nuclear morphology by Giemsa staining. Expression of wild-type p53 protein increased radiosensitivity of cell death as determined by both clonogenic and dye exclusion assays. This increase in radiosensitivity was attributable largely to apoptosis induction in addition to a small enhancement of necrosis. Interestingly neither pathway to cell death was accompanied by caspase-3 activation. On the other hand, heat-induced caspase-3 dependent apoptotic cell death without transfection was further increased by the transfection of wild-type p53. In conclusion, the introduction of wild-type p53 enhanced apoptotic cell death by X-rays or heat via different mechanisms that do or do not activate caspase-3, respectively. In addition, p53 also enhanced the X-ray-induced necrosis in M10 cells.

• Journal of Radiation Research 編集委員会の論文
• 2010-11-16

著者

• ITO Atsushi

  Department of Radiation Oncology, Hiroshima General Hospital

• NAKANO HISAKO

  Department of Laboratory Animal Science, Tokyo Metropolitan Institute of Medical Science

• SHINOHARA KUNIO

  Radiation Research Institute, Graduate School of Medicine, The University of Tokyo

• Nakano Hisako

  東京大学医学部放射線研究施設

• Nakano Hisako

  Department Of Laboratory Animal Science Tokyo Metropolitan Institute Of Medical Science

• Nakano Hisako

  Dept. Radiat. Res. Tokyo Metropolitan Inst. Med. Sci.

• Shinohara Kunio

  東京大学医学部放射線研究施設

• Shinohara Kunio

  Advanced Res. Inst. For Sci. And Engineering Waseda Univ.

• Shinohara Kunio

  Facult. Med. Univ. Of Tokyo.

• Shinohara Kunio

  Advanced Research Institute For Science And Engineering Waseda University

• Ito Atsushi

  Department Of Cardiovascular Surgery Okayama University Graduate School Of Medicine And Dentistry

• Nakano Hisako

  Department Of Food Safety Tokyo Metropolitan Institute Of Public Health

• Shinohara K

  Radiation Research Institute Graduate School Of Medicine The University Of Tokyo

• Ito Atsushi

  Department Of Nuclear Engineering School Of Engineering Tokai University

• Ito Atsushi

  Department Of Applied Chemistry College Of Engineering University Of Osaka Prefecture

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