a.母体血による胎児DNA診断(3.周産期)

概要

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Fetal cells such as nucleated red blood cells (NRBCs) have been tried to apply to non-invasive prenatal diagnosis. However we cannot still have made this non-invasive diagnosis practicable in spite of intensive studies for it. The main difficulty with this approach, however is that fetal nucleated cells are present in extremely low concentrations in maternal blood. The rarity of fetal cells in maternal circulation therefore requires the use of very sensitive methods of detection, or the use of fetal cell isolation procedures, including fluorescence activated cell sorting and micromanipulation. In addition, NRBCs isolated include cells of maternal origin. Recently, there has been much interest in the use of the noncellular portion of blood namely plasma and serum, for molecular analysis. Mainly the finding of tumor-derived DNA in the plasma and serum of cancer patients have stimulated this interest. Lo et al. first reported the usefulness of fetal DNA in maternal plasma and serum for fetal diagnosis using the detection of Y-chromosome DNA sequences derived from a male fetus.
社団法人日本産科婦人科学会の論文
2002-08-01