28 オーキシン極性移動阻害剤による黄化エンドウ芽生えの自発的形態形成の誘導 : 特にオーキシン極性移動関連遺伝子発現との関係

概要

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Etiolated pea (Pisum sativum L. cv. Alaska) seedlings have shown automorphosis in space or on a three-dimensional clinostat. Inhibitors of auxin polar transport such as 2,3,5-triiodobenzoic acid, N-(1-naphtyl)phthalamic acid and 9-hydroxyfluorene-9-carboxylic acid have also been effective to induce automorphosis-like growth on the earth, suggesting that auxin polar transport is substantially involved in automorphosis. Strenuous effort to learn between automorphosis in etiolated pea seedlings and auxin polar transport in molecular levels resulted in successful identification of putative PsPIN and PsAUX1 genes respectively encoding efflux and influx carrier proteins in plasma membrane for auxin polar transport by Southern hybridization method. PsPIN was isolated using RT-PCR techniques and cloned by TA cloning method. Significantly high homology was found on nucleotide sequences between PsPIN and AtPIN. Expression of PsPIN and PsAUX1 genes in etiolated pea seedlings was observed by RT-PCR analysis, but not by northern analysis due to possible small quantities of the expression. Extensive studies on the effects of auxin polar transport inhibitors on expression of PsPIN and PsAUX1 are now under progress. A possible role of auxin polar transport for automorphosis in higher plants will also be discussed.
植物化学調節学会の論文
2002-10-31